Different immunoelectron microscopic locations of progesterone receptor and heat shock protein 90 in the chick oviduct epithelial cells
✍ Scribed by Anu Pekki; Pentti Tuohimaa
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 107 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0739-6260
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✦ Synopsis
The intracellular locations ofthe components of the hetero-oligomeric progesterone receptor (PR), heat shock protein 90 (hsp9o) and the ligand binding component were studied immunoelectron microscopically in the chick oviduct using immunogold double labelling and peroxidase techniques with monoclonal antibodies against hsp90 (7Dcc and 4F3) and against PR (PR6 and PR 13). PR was located in the nuclei of the epithelial cells independently of the presence or absence of ligand. Cells with apical nuclei were often PR negative. After progesterone administration PR immunoreactivity decreased and the rest was seen at the margins of the hetero-and euchromatin. The nucleoli did not contain PR. Hsp9O was located in the cytoplasm as aggregates often inside small vesicles. In the apical part of the cell hsp9O was at the cisside of the Golgi complex. The nuclei contained no detectable amounts of hsp9O except for the nucleoli. Ten minutes after progesterone administration the location or immunoreactivity of hsp90 did not alter.
Thus, there seems to be a clear difference in the intracellular distribution of the PR and the hsp9o. In the epithelium there was also some cells with high levels of PR and with no or low levels of hsp90. These results raise the question whether PR is associated with hsp90 in intact cells.