✦ LIBER ✦

Different growth factors stimulate cell division of rat mammary epithelial, myoepithelial, and stromal cell lines in culture

✍ Scribed by J. A. Smith; D. P. Winslow; P. S. Rudland

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 792 KB
Volume: 119
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041190310

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✦ Synopsis

A number of single-cell-cloned cell lines have been used to examine the growth-promoting effects of putative mammotrophic agents on the various cell types in normal and neoplastic rat mammary glands. A partially purified novel pituitary-derived growth factor stimulates only cuboidal epithelial cells to divide whereas fibroblast growth factor (FGF) stimulates the growth of stromal and myoepithelial-like cells. Epidermal growth factor (EGF) has a widespread but variable growth-stimulating action, but prolactin and growth hormone are essentially inactive when added alone at a concentration of 5 pglml. Phosphoethanolamine stimulates the growth of one epithelial cell line and a derivative myoepithelial-like cell line, but is inactive on the other cell lines tested. The use of defined cloned cell lines provides a direct and reproducible assay for the identification and purification of inducers of mammary growth.

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