Different Functional Recognition of Basolateral Signals in Caco-2 and MDCK Cells
✍ Scribed by Laure Monlauzeur; Lionel Breuza; André Le Bivic
- Book ID
- 115603718
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 151 KB
- Volume
- 258
- Category
- Article
- ISSN
- 0014-4827
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## Abstract Ceftibuten uptake from the apical and basolateral side of Caco‐2 cells grown on transwells was studied. Uptake into the cells showed concentration dependent saturation. The apical transporter(s) showed a higher capacity and lower affinity for ceftibuten than the basolateral transporter(
Bidirectional transport studies were conducted to determine whether substrates of five intestinal transporters showed carrier-mediated asymmetric transport across MDCK (Madin-Darby canine kidney) cell monolayers grown under standard conditions. Drug concentrations were quantitated using liquid scint