Different effects of cyclic AMP and butyrate on eosinophilic differentiation, apoptosis and bcl-2 expression of a human eosinophilic leukemia cell line, EoL-1
✍ Scribed by Tai Guixiang; Jung Eun-Young; Horiguchi Yuji; Kawai Masahiko; Heike Toshio; Katamura Kenji; Mayumi Mitsufumi
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 823 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0278-0232
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✦ Synopsis
A human eosinophilic leukemia cell line, EoL-1. stopped proliferating at the GI phase, differentiated into eosinophilic granule-containing cells, and died by apoptosis when stimulated with dibutyryl cyclic AMP (dbcAMP). To clarify the effects of dbcAMP, the effects of butyrate and CAMP-increasing reagents, prostaglandin E, (PGE2) and forskolin. on EoL-1 cellular differentiation and apoptosis were examined and compared. PGE, and forskolin but not butyrate induced differentiation to eosinophilic granule-containing cells, suggesting that cAMP played a primary role in eosinophilic differentiation of EoL-I cells. PGE,, forskolin and butyrate, when used alone, did not induce apoptosis of EoL-I cells significantly at the concentrations used, but sequential stimulation of EoL-1 cells with the CAMP-increasing reagents and butyrate showed that butyrate induced further maturation and apoptosis of CAMP-induced eosinophilic granule-containing cells. These results showed that cAMP and butyrate have different effects on eosinophilic differentiation and apoptosis of EoL-1 cells. The CAMP-increasing reagents and butyrate also showed different effects on expression of members of the bcl-2 family; PGE, decreased bcl-2 and bax levels, whereas butyrate increased the bcl-2 level. PGE, or PGE,+ butyrate, but not butyrate alone, induced bcl-x, expression. EoL-I cells constitutively expressed Fas and anti-Fas antibody induced EoL-1 cell death, but the Fas/Fas ligand system was not involved in dbcAMP-induced EoL-1 cell apoptosis. The EoL-1 cell line is thus a useful model in which to examine differentiation and apoptosis of eosinophilic leukemia cells. ((3 1996 by John Wiley & Sons, Ltd.