Differences in surface membrane ecto-ATPase and ecto-AMPase in normal and malignant cells I. Decrease in ecto-ATPase in myeloid leukemic cells and the independent regulation of ecto-ATPase and ecto-AMPase

✍ Scribed by Benny Weiss; Leo Sachs

• Publisher: John Wiley and Sons
• Year: 1977
• Tongue: English
• Weight: 552 KB
• Volume: 93
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040930203

✦ Synopsis

Abstract

The hydrolysis of ATP and AMP by enzymes located on the external side of the plasma membrane (ecto‐ATPase and ecto‐AMPase) was studied in mouse myeloid leukemic cells, normal early myeloid cells, and normal mature granulocytes and macrophages. Nine clones of myeloid leukemic cells were used belonging to three groups that differ in their ability to be induced to differentiate by the differentiation‐inducing protein MGI. These three groups consisted of MGI^+^D^+^ that can be induced to undergo complete differentiation, MGI^+^D^+^ that can be induced to partially differentiate and MGI^−^D^−^ with no induction of differentiation. The ecto‐ATPase activity of normal early myeloid cells was similar to that of normal mature granulocytes and macrophages and higher than that of any of the leukemic cells. Among the leukemic cells, the MGI^−^D^−^ cells had the highest level of ecto‐ATPase activity.

The behaviour of ecto‐AMPase differed from that of ecto‐ATPase. Some MGI^−^D^−^ clones had a higher ecto‐AMPase activity than normal cells and MGI^+^D^−^ and MGI^+^D^+^ cells showed no detectable activity. Neither the ecto‐ATPase nor ecto‐AMPase activities changed after induction of differentiation in normal early myeloid or MGI^+^D^+^ leukemic cells. The results indicate that the myeloid leukemic cells had a decreased ability to hydrolyse external ATP, that there can be an independent regulation of ecto‐ATPase and ecto‐AMPase and that neither of these enzyme activities changed during differentiation.