Differences in patterns of TP53 and KRAS2 mutations in a large series of endometrial carcinomas with or without microsatellite instability
✍ Scribed by Elizabeth M. Swisher; Stacia Peiffer-Schneider; David G. Mutch; Thomas J. Herzog; Janet S. Rader; Alaa Elbendary; Paul J. Goodfellow
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 116 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
The correlation between tumor microsatellite instability (MSI) and the accumulation of mutations in KRAS2 and TP53 is uncertain. The authors evaluated the TP53 and KRAS2 genes for mutations in sporadic endometrial carcinomas with microsatellite instability (MSI) and matched MSI negative controls to determine whether defective DNA mismatch repair impacts the patterns of mutations in two genes known to be involved in endometrial tumorigenesis.
METHODS.
Twenty-five MSI positive endometrial tumors were matched for prognostic factors with 25 MSI negative tumors. Mutations in codon 12 and 13 of KRAS2
were assessed using a polymerase chain reaction (PCR) restriction assay. Mutations in codon 61 of KRAS2 and exons 5-8 of TP53 were evaluated using PCR amplification and single strand conformation variant (SSCV) analysis. All variants were subjected to direct DNA sequencing.