Difference in viral binding between two epstein-barr virus substrains to a spectrum of receptor-positive target cells
✍ Scribed by Alan Wells; Norio Koide; George Klein
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- French
- Weight
- 567 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Radio‐labelled Epstein‐Barr virus (EBV) was utilized in a direct binding assay to detect the presence of EBV receptors. The sensitivity of this method was affirmed by the detection of EBV‐receptors on three EBV‐carrying cell lines that have previously been reported as receptor negative. Two laboratory substrains of EBV, derived from the cell lines B95‐8 and P3HR‐1 (designated B and P virus respectively), were tested in the binding assay. The main receptor prototype adsorbed both viral strains without apparent distinction. In contrast, two lines, a Swedish EBV‐negative B‐cell lymphoma (U698) and a virus non‐producer subline of the receptor‐negative P3HR‐1 line, adsorbed P virus selectively but failed to adsorb B virus.