Difference and variation of the sef14 operon gene clusters in S. pullorum

✍ Scribed by Dr. C. H. Zhu; J. Wu; W. W. Chen; H. Musa Hassan; G. Q. Zhu

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 172 KB
• Volume: 50
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.200900262

✦ Synopsis

Abstract

SEF14 fimbriae are only found in some strains of serogroup‐D Salmonella such as S. enteritidis, suggesting that SEF14 fimbriae may affect serovar‐specific virulence traits. In this study, we found that prevalence of sefA, sefD and sefR genes in S. dublin and S. enteritidis was 100%. In 18 isolates of S. pullorum, the prevalence of sefA gene was 100%, while the prevalence of sefD and sefR genes was 38.9% (7/18), and 11 strains isolated after 1980s did not contain any gene sefD or sefR. Interestingly, among the 7 strains of S. pullorum before 1980s, the sefD sequence has a missing base pair at position 196 and caused open reading frame (ORF) shift, resulting in a stop codon (TAG) at position 71 amino acid residual (Leu of TTA at position 214–216 shift into stop codon of TAG at position 215–217). Unlike S. pullorum, all S. enteritidis and S. dublin tested could express SEF14 fimbriae in vitro. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)