✦ LIBER ✦
Diethyl pyrocarbonate: An examination of its properties in buffered solutions with a new assay technique
✍ Scribed by Shelby L. Berger
- Book ID
- 102982170
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 559 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Two assay systems for diethyl pyrocarbonate are described. The first is a spectrophotometric method that makes use of the rapid reaction with the colored compound, 5-thio-2-nitrobenzoate, to form a colorless product. The second is based on the inactivation of lactate dehydrogenase and, unlike the first. can be used in the presence of thiols. The rate of decomposition of diethyl pyrocarbonate has. been studied in several buffers at different pH values.