Dietary n-9, n-6 and n-3 fatty acids modify linoleic acid more than arachidonic acid levels in plasma and platelet lipids and minimally affect platelet thromboxane formation in the rabbit

✍ Scribed by Cristina Mosconi; Susanna Colli; Elena Tremoli; Claudio Galli

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 628 KB
• Volume: 1
• Category: Article
• ISSN: 0955-2863
• DOI: 10.1016/0955-2863(90)90044-l

✦ Synopsis

We have studied the e fleets of semisynthetic diets eontaining 5% by weight (12% of the energy) of either olive oil (70% oleic acid, OA) or corn oil (58% linoleic acid), or fish oil (Max EPA, containing about 30% eieosapentaenoic, plus docosahexaenoic, acids, and less than 2% linoleic acid), fed to male rabbits for a period of five weeks, on plasma and platelet filtty acids and platelet thromboxane formation. Aim of the study was to quantitate the absolute changes of n-6 and n-3 Jatty acid levels in plasma and platelet lipid pools after dietary manipulations and to correlate the effects on eicosanoid-precursor fatty acids with those on platelet thromboxane Jormation. The major diJ.[~'rences were fimnd when comparing the group fed fish oil and depleted linoleic acid vs the other groups. The accumulation of n-3 Jatty acids in various lipid classes was associated with modifications in the distribution of linoleie acid and arachidonic acid in different lipid pools. In platelets, maximal incorporation of n-3 J~tty acids occurred in phosphatidyl ethanolamine, which also participated in most of the total arachidonic acid reduction occurring in platelets, attd linoleie acid, more than archidonic acid, was replaced by n-3.[atty acids in various phospholipids. The archidonie acid content of phosphatidvl choline was unaffected and that of phosphatidyl inositol only marginally reduced. Thromboxane formation by thrombin stimulated platelets did not dia~]?r among the three groups, and this may be related to the minimal changes of arachidonie acid in phosphatidyl choline attd phosphatidyl inositol.