Dietary interactions of lima bean (Phaseolus lunatus) trypsin inhibitor, haemagglutinin and cyanide Part 2. Effect on pancreatic and intestinal α-amylase (EC 3.21.1.1) in growing albino rats

✍ Scribed by Aletor, V. A.

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 315 KB
• Volume: 33
• Category: Article
• ISSN: 0027-769X
• DOI: 10.1002/food.19890330515

✦ Synopsis

Dietary interactions of lima bean (Phaseolus lunatus) trypsin inhibitor, haemagglutinin and cyanide . Part 2. Effect on pancreatic and intestinal a-amylaEe (EC 3.21.1.1) in growing albino rats V. A. ALETOR A total of 108 growing albino rats was used to evaluate the dietary interactions of the major lima bean antinutritional factors trypsin inhibitor (TI), haemagglutinin (Hgg) and cyanide (CN) with respect to their effects on pancreatic and intestinal a-amylase activities. The results indicate that when fed a t the same level of activity as found in the raw lima bean (RLB) these factors had no significant (p > 0.05) influence on pancreatic a-amylase activity whether acting individually or in combination. However, when acting alone, CN appeared to depress pancreatic amylase level more than when interacting with TI or Hgg or both. Amylase activity was significantly (p < 0.01) depressed by the dietary treatments in both the small and large intestine while caecal levels were not. The most severe depression in amylase activity was elicited by the RLB diet. The haemagglutinincontaining diets appeared generally associated with lower levels of intestinal amylase activity.

From the present finding it is suggested that these factors alone cannot fully account for the magnitude of the depression of intestinal amylase activity which is contingent upon the ingestion of RLB by experimental rats.