Diels–Alder Ligation and Surface Immobilization of Proteins

✍ Scribed by Aline Dantas de Araújo; Jose M. Palomo; Janina Cramer; Maja Köhn; Hendrik Schröder; Ron Wacker; Christof Niemeyer; Kirill Alexandrov; Herbert Waldmann

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 371 KB
• Volume: 45
• Category: Article
• ISSN: 0044-8249
• DOI: 10.1002/anie.200502266

✦ Synopsis

The site-specific equipment of proteins and other biomolecules with additional functional groups for subsequent biochemical and biological investigations, for example, fluorophores, spin probes, photoactivatable groups, and affinity tags, is among the most frequently employed and important methods of research in the life sciences. A number of techniques for protein derivatization are now established. [1] Recently, in particular, aldehyde-assisted ligations, [2] native chemical ligation, [3] expressed protein ligation, [4] expressed enzymatic ligation, [5] Staudinger ligation, [6] and the application of the Huisgen azide cycloaddition [7] have been developed as powerful new methods, thereby advancing the field significantly. However, due to the multifunctionality of biomacromolecules in general and proteins in particular and the manifold applications of such techniques, there is a major and continuing demand for the development of new technology providing alternatives to the methods mentioned above.