Diazocyclopentadiene (DACP), a light sensitive reagent for the ethylene receptor in plants

✍ Scribed by E. C. Sisler; S. M. Blankenship

• Book ID: 104640798
• Publisher: Springer Netherlands
• Year: 1993
• Tongue: English
• Weight: 529 KB
• Volume: 12
• Category: Article
• ISSN: 0167-6903
• DOI: 10.1007/bf00144593

✦ Synopsis

Diazocyclopentadiene (DACP) has been shown to be an effective reagent for the ethylene receptor . Treatment of mung bean sprouts or tobacco leaves with DACP in the light or in the dark inactivates much of the ethylene binding . In the light, inactivation seems to be permanent, while in the dark, the site becomes active again after the DACP diffuses away . The compound is 10 times more effective in the light than in the dark . DACP inhibits banana ripening indicating the physiological receptor is involved . It also overcomes the inhibitory effect of ethylene on mung bean seedling growth (Km = 0 .09 ul/l E) at low ethylene levels . At high ethylene levels, an apparent high ethylene level site becomes apparent (Km = 50 tl/l E) and growth is inhibited .