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Diazirine-Based DNA Photo-Cross-Linking Probes for the Study of Protein–DNA Interactions

✍ Scribed by Uddhav Kumar Shigdel; Junliang Zhang; Chuan He


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
561 KB
Volume
120
Category
Article
ISSN
0044-8249

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An enzyme-linked microplate immunoassay for the analysis of primary DNA lesions is described. The assay principle is based on the interaction of the bacterial DNA repair proteins UvrA and UvrB with DNA and on the immunodetection of UvrB forming a stable complex with covalently modified nucleotides.