Diagnosis of human metapneumovirus by immunofluorescence-the newcastle experience
โ Scribed by C. Taylor; Y. Taha; B. Young; G. Toms; F. Fenwick; R. McGuckin
- Book ID
- 117770787
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 140 KB
- Volume
- 36
- Category
- Article
- ISSN
- 1386-6532
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract Over two winters in Newcastle upon Tyne, respiratory secretions, negative by immunofluorescence staining for other respiratory viruses, were tested for the presence of human metapneumovirus (HMPV) by RTโPCR. In the second winter, specimens were also tested by immunofluorescence staining
Rabbit antisera were prepared against coronavirus strains 229E and OC43 and used successfully to detect viral antigen in epithelial cells shed from the nasopharynx of symptomatic volunteers who had received coronavirus inocula three to four days before. The same serologic reagents were applied to na