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Diagnosis of bladder cancer by analysis of the allelic loss of the p53 gene in urine samples using blunt-end single-strand conformation polymorphism

✍ Scribed by Kokichi Sugano; Masakazu Tsutsumi; Yuki Nakashima; Kensei Yamaguchi; Hisanao Ohkura; Tadao Kakizoe; Takao Sekiya

Publisher: John Wiley and Sons
Year: 1997
Tongue: French
Weight: 64 KB
Volume: 74
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19970822)74:4<403::aid-ijc7>3.0.co;2-z

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✦ Synopsis

The novel approach of blunt-end single-strand conformation polymorphism (SSCP) has been applied in the analysis of urine samples from bladder-cancer patients for detecting loss of heterozygosity (LOH) of 3 polymorphic markers in the p53 gene. Of the 28 urine samples examined by SSCP analysis of blunt-ended DNA fragments using a fluorescence-based automated sequencer, 16 were informative in more than 1 of the 3 polymorphic markers at the p53 locus and 8 (50.0%) showed allelic loss of the p53 gene. In analysis of resected tumor tissues, LOH of the p53 gene was detected in 8 of 8 informative samples (100%) with T1 and higher stages and/or Grade 2 and Grade 3 tumors, while it was detected in 6 (75.0%) urine samples obtained from these 8 patients. This new diagnostic modality enables sensitive detection of tumor cells in urine samples and would be applicable for diagnostic bladder cancer with invasive character.

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