Diagnosis of a case of acute chloroquine poisoning using 1H NMR spectroscopy: characterisation of drug metabolites in urine

✍ Scribed by Susanna Maschke; Nathalie Azaroual; Jean-Michel Wieruszeski; Guy Lippens; Michel Imbenotte; Daniel Mathieu; Gaston Vermeersch; Michel Lhermitte

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 245 KB
• Volume: 10
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/(sici)1099-1492(199709)10:6<277::aid-nbm479>3.0.co;2-m

✦ Synopsis

Analysis of biological fluids by proton nuclear magnetic resonance spectroscopy ( 1 H NMR) is a promising tool in clinical biology. We have used this method for a rapid toxicological screening in the case of a suicide attempt. A urine sample was analysed at 300 and 600 MHz by 1D and 2D sequences (J-resolved and TOCSY) in a short experimental time. Quantification was realized by peak integration of the 1D spectrum. The results showed the presence of chloroquine and its major metabolite monodesethylchloroquine at concentrations of 462 and 140 mg/ L, respectively. Ethanol was also detected in the spectrum. It can be concluded that 1 H NMR provides many advantages as a tool for clinical diagnosis in a case of acute intoxication.