Diagnosis and quantitative evaluation of
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Elisabetta Manaresi; Giorgio Gallinella; Elisa Zuffi; Francesca Bonvicini; Maria
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Article
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2002
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John Wiley and Sons
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English
β 134 KB
## Abstract A realβtime PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent doubleβstranded DNA binding dye SYBR Green I. With an optim