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Diagnosis and follow-up of Bartonella henselae infection in the spleen of an immunocompetent patient by real-time quantitative PCR

✍ Scribed by Liberto, M. C.; Matera, G.; Lamberti, A. G.; Quirino, A.; Barreca, G. S.; Marascio, N.; Baudi, F.; Caroleo, B.; Staltari, O.; Foca, A.


Book ID
120160943
Publisher
Society for General Microbiology
Year
2013
Tongue
English
Weight
97 KB
Volume
62
Category
Article
ISSN
0022-2615

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## Abstract A real‐time PCR assay was developed for quantitative detection of B19 DNA in clinical serum samples. The assay was carried out using a LightCycler instrument and product formation was monitored continuously with the fluorescent double‐stranded DNA binding dye SYBR Green I. With an optim