Diagnosis and characterization of insecticide-insensitive acetylcholinesterase in three populations of the sweetpotato whitefly Bemisia tabaci

✍ Scribed by Anthony, Nicola M.; Brown, Judith K.; Feyereisen, Rene; Ffrench-Constant, Richard H.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 357 KB
• Volume: 52
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(199801)52:1<39::aid-ps669>3.0.co;2-f

✦ Synopsis

A biochemical approach was used to characterize acetylcholinesterase (AChE) insecticide insensitivity in several sweetpotato whiteÑy (Bemisia tabaci ; SPW) populations. Discriminating doses of insecticide were established to di †erentiate between sensitive and insensitive SPW strains and to genotype individual whiteÑy. This technique was then used to examine the frequency of insensitive AChE alleles in several SPW populations and to isolate a line homozygous for insensitive AChE from a heterogenous B-type population. Inheritance of putative altered AChE genotypes was consistent with the proposed haplo-diploid status of B. tabaci. This biochemical diagnostic was also employed to determine the role of insensitive AChE in the observed resistance proÐles of several laboratory populations subjected to di †erent selection regimes. In keeping with previous studies on insecticide resistance in SPW, resistance does not appear to be uniquely associated with the B-type but rather with SPW populations found in crop systems.

1998 SCI.