Signal transduction pathways controlling tumor cell locomotion are not yet well understood. We have studied the role of protein kinase C (PKC)-dependent protein phosphorylation associated with changes in cell shape and locomotor activity of Walker carcinosarcoma cells in culture. We show that the in
Diacylglycerols and the protein kinase inhibitor H-7 suppress cell polarity and locomotion of walker 256 carcinosarcoma cells
โ Scribed by H. U. Keller; A. Zimmermann; V. Niggli
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- French
- Weight
- 644 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0020-7136
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โฆ Synopsis
Publication of the International Union Against Cancer Publication de I'Union lnternationale Contre le Cancer
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Treatment with low (nanomolar) concentrations of phorbol-12-myristate-13-acetate (PMA) for 5 to 30 min suppresses locomotion of Walker 256 carcinosarcoma cells, suggesting that activation of protein kinase C (PKC) is a stop signal for tumor cell locomotion. We have compared the effects of PMA on cel
The putative role of cellular or solvent volume in protrusive activity and locomotion has been investigated in blebbing Walker carcinosarcoma cells using hypertonic media. Blebbing, locomotion, and cell polarity are completely suppressed by 0.2 M sorbitol. The response occurs in two steps. In a firs