Di-tert-butylhydroxylated flavonoids protect endothelial cells against oxidized LDL-induced cytotoxicity

✍ Scribed by Christophe Furman; Jonathan Lebeau; Jean-Charles Fruchart; Jean-Luc Bernier; Patrick Duriez; Nicole Cotelle; Elisabeth Teissier

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 205 KB
• Volume: 15
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/jbt.10003

✦ Synopsis

Abstract

The protective effect of di‐tert‐butylhydroxylated flavonoids (chalcones and arylidenes) against minimally oxidized LDL (mO‐LDL)‐induced cytotoxicity was studied in cultured bovine aortic endothelial cells. Most of the tested compounds decreased aldehydes formation in medium containing mO‐LDL, but their capacity to inhibit LDL oxidation in the cellular medium was not sufficient to totally reduce the cellular toxicity of mO‐LDL. Most of the tested flavonoids improved the integrity of cells exposed to mO‐LDL, whereas butylated hydroxytoluene was ineffective and quercetin worsened the toxicity of mO‐LDL. Moreover these flavonoids induced an increase in GSH cellular levels and their protective effects might be because of their inability to reduce metal ion. Arylidene 6 substituted at position 7 by a hydroxyl group was the most potent compound. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:270–278, 2001