Dextran-based coating system for the immobilization of cell adhesion promoting molecules on titanium surfaces
✍ Scribed by M. Dubs; J. Weisser; R. Linke; A. Pfuch; D. Imhof; M. Schnabelrauch
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 283 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0933-5137
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✦ Synopsis
Abstract
Immobilization of adhesive peptides interacting with cellular integrin receptors onto metallic implant surfaces represents a promising approach to improve osseointegration of implants into the surrounding tissue. In the present study, a functional dextran‐based coating system consisting of an amino titanate adhesion promoter with dendritic structure and a carboxymethyl dextran was established to bind an RGD‐containing adhesive peptide via a selective coupling methodology onto titanium surfaces. The three‐step reaction procedure was characterized by X‐ray photoelectron spectroscopy. In cell adhesion experiments it could be demonstrated that dextran coatings containing immobilized RGD promote attachment and spreading of fibroblast and pre‐osteoblastic cells compared to native as well as CMD‐coated titanium surfaces without RGD. The direct attachment of the RGD sequence to the metal surface via the amino titanate adhesion promoter did not increase pre‐osteoblastic cell spreading, whereas coupling of RGD to the polymeric carboxymethyl dextran layer slightly enhanced spreading of the cells.