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Dexamethasone regulation of marrow stromal-derived osteoblastic cells

✍ Scribed by A. Fried; D. Benayahu

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 675 KB
Volume: 62
Category: Article
ISSN: 0730-2312
DOI: 10.1002/(sici)1097-4644(19960915)62:4<476::aid-jcb5>3.0.co;2-j

No coin nor oath required. For personal study only.

✦ Synopsis

The clonal subtypes of cells in the osteogenic family represented by fibroblastoid , and mature osteoblastic MBA-15.6 cells were used to study the effects of glucocorticoid (dexamethasone). The role of dexamethasone was monitored on cell attachment when plated on various protein substrata (BSA, collagen I, and Matrigel). A 24 h exposure of the cells to 1 0-6 M or 1 O-' M dexamethasone differential affects their attachment preference. MBA-15.33 and MBA-15.4 cells increased their attachment capability on collagen I, while MBA-15.6 cells' attachment was inhibited. Pretreatment with M) dexamethasone caused an increase in attachment on Matrigel by MBA-15.33 cells and to less extent by MBA-15.4 cells. Additionally, measurements of two enzymatic activities were monitored; one is alkaline phosphatase (ALK-P), and the second is neutral endopeptidase (CDI O/NEP). cells were exposed to dexamethasone or to various growth factors (bone morphogenic protein (BMP-2 and BMP-3), TGFP, and IGF-I). In some experiments, pretreatment of cells by dexamethasone was followed by exposure to the growth factors. The cells' challenged cellular responses were not uniform and revealed a differential pattern when their ALK-P and CD1 O/NEP enzymatic activities were measured.

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