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Dexamethasone blocks nerve growth factor induction of nerve growth factor receptor mRNA in PC12 cells

✍ Scribed by P. J. Foreman; G. Taglialatela; G. R. Jackson; J. R. Perez-Polo

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 528 KB
Volume: 31
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490310108

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✦ Synopsis

Abstract

Glucocorticoids and nerve growth factor (NGF) have been shown to have antagonistic effects on chromaffin cells in vivo. Here we determined the effect of the synthetic glucocorticoid, dexamethasone, on levels of mRNA for the nerve growth factor receptor (NGFR) in rat PC12 pheochromocytoma cells. Following administration of dexamethasone (1 μM) there is a decline in NGFR mRNA expression. More importantly, administration of dexamethasone appears to block the NGF‐mediated induction of NGFR when both agents are administered simultaneously. These data support the hypothesis that glucocorticoids and NGF act in opposition in determination of the phenotype of chromaffin cells.

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