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Developmental regulation of 14-3-3 ϵ isoform in rat heart

✍ Scribed by Sharon C.W. Luk; Sai-ming Ngai; Stephen K.W. Tsui; Kwok-keung Chan; Kwok-pui Fung; Cheuk-yu Lee; Mary M.Y. Waye

Book ID
101262092
Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
108 KB
Volume
68
Category
Article
ISSN
0730-2312

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✦ Synopsis

Human heart cDNA sequencing yielded a cDNA clone that is similar in DNA and amino acid sequences to that of mouse 14-3-3 ⑀ isoform. The 6xHis-tagged H1433⑀ recombinant protein was expressed in Escherichia coli and its size was approximately 30 kDa. From Northern blot results with human multiple tissues, human skeletal muscle was found to have the highest level of h1433⑀ mRNA expression, whereas Northern blots of human cancer cell lines detected the highest mRNA level of h1433⑀ in colorectal adenocarcinoma SW480. The protein expression level of h1433⑀ and Raf-1 is found to be regulated coordinately during rat heart development, and their protein expression was highest from 14.5 to 16.5 days postcoitum.

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