Developmental physiology of cestodes XVI. Effects of certain excretory products on incorporation of3H-thymidine into DNA ofHymenolepis diminuta
✍ Scribed by Insler, Gayle Dranch ;Roberts, Larry S.
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 524 KB
- Volume
- 211
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Succinate and acetate, which are excreted by Hymenolepis diminuta (Cestoda: Cyclophyllidea), account for about half the inhibition of DNA synthesis observed when the worms are incubated in a “worm conditioned saline” (WCS). Succinate is probably responsible for the bulk of the inhibition, while lactate, which is also excreted by the worms, is inactive. Ammonia and urea at concentrations present in the WCS do not inhibit incorporation of thymidine into DNA. Incubation in WCS or 12 mM succinate, for more than 1 hr inhibits glucose uptake by the worms, but the effect is reversible; therefore, the inhibition is not a result of permanent damage. Concentrations of succinate found in WCS appear physiologic since they agree well with levels found in the small intestines of rats infected with high density populations of H. diminuta.