We employed whole-mount in situ hybridization and immunohistochemistry to study the spatial pattern of hsp30 gene expression in normal and heatshocked embryos during Xenopus laevis development. Our findings revealed that hsp30 mRNA accumulation was present constitutively only in the cement gland of
Developmental expression patterns of the zebrafish small heat shock proteins
β Scribed by Martha Marvin; Devon O'Rourke; Tomoki Kurihara; Courtney E. Juliano; Krista L. Harrison; Lara D. Hutson
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 368 KB
- Volume
- 237
- Category
- Article
- ISSN
- 1058-8388
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In this study, we analyzed the response of the temperate, shallow-water gorgonian, Leptogorgia virgulata, to temperature stress. Proteins were pulse labeled with (35)S-methionine/cysteine for 1 h to 2 h at 22 degrees C (control), or 38 degrees C, or for 4 h at 12.5 degrees C. Heat shock induced synt
To elucidate the potential role of the hsp70 gene family in developmental processes in vertebrates, we chose to study the expression of one of these genes in zebrafish. A zebrafish gastrula cDNA library was screened with a Pleurodeles waltl hsp70 cDNA probe. A 2.3-kb cDNA was thus isolated and seque
In normal muscle, 65-kd heat shock proteins (hsp) were detected on capillary endothelial cells, the mural elements of larger vessels, and some intracellular organelles, probably mitochondria. In the inflammatory myopathies, the 65-kd hsp were detected on inflanunaxory cells, degenerating and regener
## Abstract There are several reports describing participation of small heat shock proteins (sHsps) in cellular protein quality control. In this study, we estimated the endoplasmic reticulum (ER) stressβinduced response of Hsp27 and Ξ±Bβcrystallin in mammalian cells. Treatment targeting the ER with