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Developmental changes in the activity and cellular localization of hepatic casein kinase II in the rat

โœ Scribed by Philip A. Gruppuso; Joan M. Boylan

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
749 KB
Volume
58
Category
Article
ISSN
0730-2312

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โœฆ Synopsis

The activity and cellular localization of hepatic casein kinase I1 (CKII) was examined during late fetal development in the rat. Cultured fetal hepatocytes displayed constitutive CKll activity which was not further activated by growth factor exposure. Similarly, fetal liver CKll showed approximately fivefold greater activity than adult liver. The fetal hepatic activity was, to a large degree, localized to a nuclear fraction. Postnuclear cytosol preparations from fetal and adult liver showed similar CKll activity. In all cases, FPLC ion exchange chromatography followed by Western immunoblotting showed that immunoreactive CKll coincided with kinase activity. However, parallel determinations of CKll activity and immunoreactive CKll levels showed a higher (fiveto sixfold) CKll specific activity in nuclear extracts compared to cytosol. In summary, fetal hepatic CKll demonstrates coincident nuclear localization and activation. We hypothesize that the regulation of hepatic CKll is relevant to the mitogen-independent proliferation displayed by fetal rat hepatocytes.

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