Developmental changes in capacitative Ca2+ entry in mouse mammary epithelial cells

✍ Scribed by Junm Ichikawa; Kishio Furuya; Seiji Miyata; Toshihiro Nakashima; Toshikazu Kiyohara

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 297 KB
• Volume: 18
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(200006)18:2<147::aid-cbf864>3.0.co;2-w

✦ Synopsis

Developmental changes in capacitative Ca 1¦ entry and Ca 1¦ release from intracellular stores were measured using fura!1 ~uorescence method during the pregnancy period "day 2Ð07# in mouse mammary epithelial cells[ Ca 1¦ release was identi_ed with the transient intracellular Ca 1¦ "ðCa 1¦ Ł i # increase induced by thapsigargin addition in a Ca 1¦ !free solution[ Capacitative Ca 1¦ entry was measured by the transient ðCa 1¦ Ł i increase induced by re!addition of extra! cellular Ca 1¦ after depletion of Ca 1¦ stores by thapsigargin[ The capacitative Ca 1¦ entry was greatest at the early stage of pregnancy "i[e[ day 2 of pregnancy# and decreased as pregnancy progressed\ while Ca 1¦ release remained unchanged throughout the developmental stages[ These _ndings indicate that in contrast to Ca 1¦ release\ a close correlation exists between capacitative Ca 1¦ entry and pregnancy!induced development in mammary epithelial cells[