Development of two-cell mouse embryos in protein-free and protein-supplemented media
โ Scribed by Framila V. Dandekar; Robert H. Glass
- Publisher
- Springer US
- Year
- 1990
- Tongue
- English
- Weight
- 615 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1573-7330
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๐ SIMILAR VOLUMES
Proteins secreted by mouse blastocysts developing in vitro were compared to those from blastocysts developing in utero to determine if a simple medium supporting blastocyst development also supports secreted protein expression. In-vivo embryos were collected on days 3,4, or 5 of pregnancy and incuba
## Abstract Spectral variants of green fluorescent protein are widely used in live samples for a broad range of applications: from visualization of protein interactions, through following gene expression, to marking particular cells in complex tissues. Higher wavelength emissions (such as red) are
Please note the correction to the above article: The red fluorescent protein used for the results in the above article was erroneously designated ''DsRed.T3.'' The correct name of the variant is DsRed-MST. DsRed-MST differs from DsRed.T3 in two amino acids. It has Alanine at positions 145 and 217. W
## Abstract The effect of inhibitors of RNA synthesis (Cordycepin, Actinomycin D) and protein synthesis (Cycloheximide) on the development and growth of mouse blastocysts in vitro was explored. Blastocysts exposed in vitro for 24 hours to 50 ฮผg/ml Cordycepin, 0.005 ฮผg/ml Actinomycin D, or 0.1 ฮผg/ml