Development of specifications for labelled mucleotides and related compounds, specific activity measurements dy mass spectrometry

✍ Scribed by Gerald Goldstein; W. T. Rainey

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: French
• Weight: 316 KB
• Volume: 9
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2590090422

✦ Synopsis

Abstract

The National Academy of Science‐National Research Council Committee on Specifications and Criteria for Biochemical Compounds is considering the problems involved in preparing specifications for labelled biochemicals. The Subcommittee on Nucleotides and Related Compounds has examined methods for specific activity measurement. We find that the specific activity of ^14^C‐labelled purines and pyrimidines can be determined accurately and precisely by mass spectrometry. Moreover, from the known fragmentation patterns and the isotopic abundance of carbon‐14 in the fragments, the position of the labelled atoms can often be established and the labelling pattern in “uniformly labelled” compunds described. In two of the five compounds tested, the specific activity determined by mass spectrometry was 20% higher than the quoted value.