Development of specific antisera against 9-desglycinamide-8-arginine vasopressin by site-specific immunization
✍ Scribed by Joost B. M. M. Van Bree; Jan W. Van Nispen; J. Coos Verhoef; Douwe D. Breimer
- Book ID
- 102408134
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 421 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
Four specific and sensitive antisera against 9-desglycinamide-8-arginine vasopressin (DGAVP) were obtained by means of site-specific immunization in rabbits, using a Keyhole Limpet haemocyanin-DGAVP conjugate synthesized by a specific reaction of a thiol function with a maleimide group. Compared with conventionally raised antisera, the cross-reactivity with endogenous arginine vasopressin (AVP) and vasopressin-derived fragments was substantially lower. Interassay variability was 18.1% at maximum. Possible applicability of the new antisera in pharmacokinetic studies of DGAVP with respect to blood-brain barrier transport was tested by means of a pilot study in the rat.