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Development of Quantum Dots Modified Acetylcholinesterase Biosensor for the Detection of Trichlorfon

✍ Scribed by Xiao-Hua Li; Zonghong Xie; Hong Min; Chenxin Li; Meichuan Liu; Yuezhong Xian; Litong Jin

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
157 KB
Volume
18
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

Poly (N‐vinyl‐2‐pyrrolidone) (PVP)‐capped CdS quantum dots (QCdS‐PVP) was synthesized with CdCl~2~ and Na~2~S in the presence of PVP. QCdS‐PVP has been used for the immobilization and stabilization of the acetylcholinesterase (AChE). The electrocatalytic activity of QCdS‐PVP leads to a greatly improved electrochemical detection of the enzymatically generated thiocholine product, and higher sensitivity and stability. The GCE/QCdS‐PVP/AChE biosensor was used for the detection of organophosphate pesticides (OPs), such as trichlorfon. The sensor performance, including pH and inhibition time, was optimized with respect to operating conditions. Under the optimal conditions, the biosensor was used to measure as low as 12 ppb trichlorfon with a 5‐min inhibition time.

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Comparison of immobilization methods for
Comparison of immobilization methods for the development of an acetylcholinesterase biosensor
✍ Kathrin Stein; Georg Schwedt πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 773 KB

Different methods for the immobilization of acetylcholinesterase (AChE), viz, co-cross-linking at the surface of a pH electrode, co-cross-linking in a membrane and trapping in a polyacrylamide membrane, are described The immobilization products are compared with respect to the response times, the re