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Development of quantitative enzymatic method and its validation for the assay of glucose in human serum

โœ Scribed by Padmarajaiah Nagaraja; Honnur Krishna; Anantharaman Shivakumar; Ashwinee K. Shrestha

Book ID
118431662
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
294 KB
Volume
45
Category
Article
ISSN
0009-9120

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Development of an enzymatic method for t
Development of an enzymatic method for the assay of serum magnesium using phosphoglucomutase and glucose-6-phosphate dehydrogenase
โœ Kenji Suzuki; Yuzo Kayamori; Yoshiaki Katayama ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 353 KB

We developed an assay for serum magnesium (Mg) by coupling phosphoglucomutase (EC 2.7.5.1) with glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The kinetic generation of NADPH by the action of the above two enzymes upon glucose-l-phosphate (Gl-P) and glucoso-l,6-diphosphate (G-1,6-P) was proportion