Development of protein-deficient serum-free culture media for adherent cell lines suited for large scale cultivation

Development and characterization of a ce

Development and characterization of a cell line for large-scale, serum-free production of recombinant adeno-associated viral vectors

✍ Deborah Farson; Thomas C. Harding; Lucy Tao; Jun Liu; Sandra Powell; Vishalini V 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 448 KB

## Abstract ## Background One of the major limitations to the use of adeno‐associated virus (AAV) vectors for gene therapy has been the difficulty in producing enough vector to supply a clinical trial. More than 20 000 roller bottles may be required to generate AAV by the traditional transient tra