Development of polarity in cerebellar granule neurons

✍ Scribed by Powell, Sharon K. ;Rivas, Rodolfo J. ;Rodriguez-Boulan, Enrique ;Hatten, Mary E.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 290 KB
• Volume: 32
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199702)32:2<223::aid-neu7>3.0.co;2-a

✦ Synopsis

Axon formation in developing cerecell surface distribution of a specific class of endogebellar granule neurons in situ is spatially and temponous glycoproteins, those linked to the membrane by rally segregated from subsequent neuronal migration a glycosylphosphatidyl inositol (GPI) anchor, was also and dendrite formation. To examine the role of local examined. The GPI-anchored protein, TAG-1, which environmental cues on early steps in granule cell difis segregated to the parallel fiber axons in situ, was ferentiation, the sequence of morphologic developfound exclusively on granule cell axons in vitro; howment and polarized distribution of membrane proever, two other endogenous GPI-anchored proteins teins was determined in granule cells isolated from were found on both the axonal and somatodendritic contact with other cerebellar cell types. Granule cells domains. These results demonstrate that granule cells cultured at low density developed their characteristic develop polarity in a cell type-specific manner in the axonal and dendritic morphologies in a series of disabsence of the spatial cues of the developing cerebellar crete temporal steps highly similar to those observed cortex.