Development of highly sensitive and specific HPLC assay for plasma morphine using direct injection technique and post-column derivatization
✍ Scribed by Samy Emara
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 102 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0269-3879
No coin nor oath required. For personal study only.
✦ Synopsis
An extremely simple, rapid and reproducible analytical method was developed for the determination of morphine in human plasma using a high performance liquid chromatography utilizing a column-switching technique and protein-coated precolumn. Morphine from plasma (500 L) was preconcentrated on the protein-coated precolumn without sample pre-treatment. This column acted at the same time as a clean up device. The drug was transferred on-line to the analytical column followed by post-chromatographic derivatization and fluorimetric detection. Post-column derivatization was based on the oxidative dimerization of morphine to fluorescent pseudomorphine by potassium hexacyanoferrate (III). The average morphine recoveries over a concentration range of 10 to 100 ng/mL ranged from 94.84 to 100.70%, and relative standard deviations ranged from 1.36 to 2.13%.