Development of hamster two-cell embryos in the isolated mouse oviduct in organ culture system
β Scribed by Minami, N. ;Bavister, B. D. ;Iritani, A.
- Book ID
- 111663326
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1988
- Weight
- 380 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0148-7280
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Successful development of porcine embryos from the one-cell stage to the blastocyst stage has been accomplished using mouse oviducts in organ culture. One-cell embryos were transferred to mouse oviducts maintained in organ culture and were cultured for 6 days. Control embryos from each donor pig wer
The effect of increasing the embryo:medium volume ratio on overcoming the hamster two-cell block was examined. Two-cell golden hamster embryos from each superovulated female were cultured in microdrops (estimated at 0.75 microliter) or 100 microliter macrodrops of chemically defined medium (modified