## Abstract We have developed a chemical derivatization scheme for primary amines that couples the fast kinetic properties of __o__βphthaldialdehyde (OPA) with the photophysical properties of visible, high quantum yield, fluorescent dyes. In this reaction, OPA is used as a crossβlinking reagent in
Development of fluorescence between o-phthaldialdehyde and thiols
β Scribed by P.C. Jocelyn; A. Kamminga
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 275 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
β¦ Synopsis
An assay for GSH which depends on the development of fluorescence after treating the thiol with OPT2 at pH 8 was devised by Cohn and Lyle (1) and further studied by McNeil and Beck (2). Amounts of GSH as low as lo-lo mole were determined. Other compounds including cysteine, homocysteine and ergothioneine were found to give little or no fluorescence
π SIMILAR VOLUMES
The enzyme N5-methyltetrahydrofolate-homocysteine methyltransferase (methionine synthase, EC 2.1.1.13) catalyzes the conversion of homocysteine to methionine in the presence of a reducing system. N5-Methyltetrahydrofolate serves as a methyl donor in this reaction. An assay for the enzyme is describe