𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Development of fluorescence between o-phthaldialdehyde and thiols

✍ Scribed by P.C. Jocelyn; A. Kamminga


Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
275 KB
Volume
37
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis


An assay for GSH which depends on the development of fluorescence after treating the thiol with OPT2 at pH 8 was devised by Cohn and Lyle (1) and further studied by McNeil and Beck (2). Amounts of GSH as low as lo-lo mole were determined. Other compounds including cysteine, homocysteine and ergothioneine were found to give little or no fluorescence


πŸ“œ SIMILAR VOLUMES


Design, characterization, and utilizatio
✍ Suminda Hapuarachchi; Craig A. Aspinwall πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 414 KB

## Abstract We have developed a chemical derivatization scheme for primary amines that couples the fast kinetic properties of __o__‐phthaldialdehyde (OPA) with the photophysical properties of visible, high quantum yield, fluorescent dyes. In this reaction, OPA is used as a cross‐linking reagent in

A nonradioactive assay fo N5-methyltetra
✍ Alexis Garras; Rune Djurhuus; Benedicte Christensen; Johan R. Lillehaug; Per Mag πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 756 KB

The enzyme N5-methyltetrahydrofolate-homocysteine methyltransferase (methionine synthase, EC 2.1.1.13) catalyzes the conversion of homocysteine to methionine in the presence of a reducing system. N5-Methyltetrahydrofolate serves as a methyl donor in this reaction. An assay for the enzyme is describe