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Development of duplex real-time RT-PCR based on Taqman technology for detecting simultaneously the genome of pan-enterovirus and enterovirus 71

✍ Scribed by Hwang, Seoyeon; Kang, Byunghak; Hong, Jiyoung; Kim, Ahyoun; Kim, Hyejin; Kim, Kisang; Cheon, Doo-Sung


Book ID
121748136
Publisher
John Wiley and Sons
Year
2013
Tongue
English
Weight
69 KB
Volume
85
Category
Article
ISSN
0146-6615

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## Abstract A two‐step reverse transcriptase TaqMan™ duplex PCR (RT‐PCR) assay was developed using the ABI 7700 Sequence Detection System for the detection of enterovirus (EV) and parechovirus type 1 and 2 (PEV) RNA from samples of cerebrospinal fluid (CSF) and throat swabs. Using sequence‐specific