Development of dopamine neurotransmission and uptake inhibition in the caudate nucleus as measured by fast-cyclic voltammetry
✍ Scribed by Sara R. Jones; Brian P. Bowman; Cynthia M. Kuhn; R. Mark Wightman
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 299 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0887-4476
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✦ Synopsis
The ability to measure stimulated dopamine efflux in real time using fast-scan cyclic voltammetry (FSCV) has allowed for the assessment of the kinetics of dopamine (DA) release and uptake. Using this technique in brain slices, regional differences in dopamine efflux and the effects of cocaine (COC) have been detected that have not been discovered using other methods (Jones et al., 1995a,b; Wieczorek and Kruk, 1994). Interest in the development of the DA transporter (DAT) has evolved from growing concerns over the effects of gestational exposure to COC. Behavioral studies in rats have shown that COC stimulates behavior at very early ages (Bowman and Kuhn, in press;Spear and Brick, 1979) although binding studies indicate that the expression of DAT in the caudate nucleus (CN) rises rapidly towards adult levels only during the second and third weeks of life (Rao et al., 1991). The goal of the present study was to assess age-related differences in release and uptake of DA in the CN and to examine the effects of the DAT inhibitor COC.
Adult male Sprague-Dawley rats (200-400 g, Charles River, Wilmington, MA) and dams with litters (10-12 day old) were given ad libitum food and water, and received care in accordance with the Guide for Care and Use of Laboratory Animals (NIH publication 865-23, Bethesda, MD) and approved by IACUC of Duke University and the University of North Carolina. CN slices (about 400 pm thick) were prepared with a Lancer Vibratome (Technical Products International, Inc., St. Louis, MO) and superfused with a Kreb's buffer (Jones et al., 1995a) in a recording chamber for 30-45 min before recordings were made. Carbon-fiber (Thornel P-55,5 pm radius, Amoco, Greenville, SC) microelectrodes were prepared as described previously (Kawagoe et al., 1993), and coated with Nafion (2.5% wh). Electrodes