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Development of Cell-Based High-Throughput Assays for the Identification of Inhibitors of Receptor Activator of Nuclear Factor-Kappa B Signaling

✍ Scribed by Ashley, Jason W.; McCoy, Erin M.; Clements, Daniel A.; Shi, Zhenqi; Chen, Taosheng; Feng, Xu


Book ID
120749962
Publisher
Mary Ann Liebert
Year
2011
Tongue
English
Weight
750 KB
Volume
9
Category
Article
ISSN
1540-658X

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Quantitative, High-Throughput Cell-Based
✍ Thelma S. Angeles; Jonathan S. Lippy; Shi X. Yang πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 130 KB

Two quantitative, high-throughput cell-based assays for evaluating inhibitors of NGF-stimulated trkA phosphorylation in trkA-transfected NIH3T3 cells have been established. Both assays involve capture of the trkA receptor from cell lysates in microtiter plates coated with an anti-trk antibody. The a