Development of C4photosynthesis in sugar cane: Changes in properties of phosphoenolpyruvate carboxylase during greening

✍ Scribed by M. Bruce Goatly; James Coombs; Harry Smith

• Publisher: Springer-Verlag
• Year: 1975
• Tongue: English
• Weight: 589 KB
• Volume: 125
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00388869

✦ Synopsis

Phosphoenolpyruvate (PEP) carboxylase extracted from etiolated and greening sugar cane {a Saccharum hybrid) displayed different properties in terms of DEAE-cellulose elution profile and activation by glucose-6-phosphate. During the first 20 hours of greening, no increase in extractable PEP carboxylase activity was observed, but ionic and allosteric properties of the enzyme changed, becoming similar to those found for ~he enzyme from light-grown cane. Density labelling studies with deuterium oxide provided no evidence for either de novo synthesis or turnover of the enzyme during this period, or during the following 24 hours of greening. These results are discussed in relation to enzyme control mechanisms and the development of the C 4 pathway in photosynthesis.