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Development of buffers for fast semidry transfer of proteins

✍ Scribed by Garić, Dušan; Humbert, Laure; Fils-Aimé, Nadège; Korah, Juliana; Zarfabian, Yasaman; Lebrun, Jean-Jacques; Ali, Suhad

Book ID
121292254
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
366 KB
Volume
441
Category
Article
ISSN
0003-2697

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Optimization of buffer solutions for pro
Optimization of buffer solutions for protein crystallization
✍ Gosavi, Rajendrakumar A. ;Mueser, Timothy C. ;Schall, Constance A. 📂 Article 📅 2008 🏛 International Union of Crystallography 🌐 English ⚖ 264 KB

Increasing the solubility of protein stock solutions to above that in a standard chromatography buffer (50 mM Tris-HCl pH 7.5, 100 mM NaCl) led to an increase in the number of crystallization conditions for ten globular proteins subjected to two crystal screens: the Index and Precipitant/Precipitant