Development of anin VitroTranslation System from Wheat Germ That Is Dependent upon the Addition of Eukaryotic Initiation Factor 2
✍ Scribed by Lisa A. Benkowski; Joanne M. Ravel; Karen S. Browning
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 84 KB
- Volume
- 232
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
required as a source of aminoacyl-tRNA synthetases, elongation factors, and termination factors, but also contain eIF-2 (9,10). The amount of an ammonium sulfate fraction (40-70%) added to the wheat germ assay Development of an in Vitro Translation System system contains sufficient amounts of eIF-2 to obtain from Wheat Germ That Is Dependent upon the optimal translation (10). The only in vitro assay cur- Addition of Eukaryotic Initiation Factor 2 rently available for measuring the activity of eIF-2 is based on the ability of eIF-2 to form a ternary complex Lisa A. Benkowski, 1 Joanne M. Ravel, formation with GTP and [ 35 S]Met-tRNA i . This assay does not measure the overall activity of eIF-2 in the and Karen S. Browning 2 initiation process.