✦ LIBER ✦

Development of an inflammation-inducible gene expression system using helper-dependent adenoviral vectors

✍ Scribed by Tianyao Yang; Rongqi Duan; Huibi Cao; Benjamin H. Lee; Chun Xia; Zhijie Chang; A. Keith Tanswell; Jim Hu

Book ID: 102340757
Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 393 KB
Volume: 12
Category: Article
ISSN: 1099-498X
DOI: 10.1002/jgm.1501

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✦ Synopsis

Abstract

Background

Clinical studies have shown that gene therapy is a promising approach for treating such genetic diseases as the eye disease, Leber's congenital amaurosis. Development of gene therapy approaches for treating chronic inflammatory diseases is, however, more challenging because it requires the production of anti‐inflammatory molecules at the diseased tissues only when they are needed.

Methods

We designed such a system by modifying the human interleukin (IL)‐6 gene promoter to direct transgene expression and delivered the system into cultured cells as well as mouse lungs using a helper‐dependent adenoviral vector.

Results

We have demonstrated both in vitro and in vivo that the reporter LacZ or human IL‐10 gene can be induced by inflammatory stimuli.

Conclusions

The results obtained indicate that the inflammation inducible gene expression system based on the modified human IL‐6 gene promoter has the potential to be used for developing gene therapy for treating inflammatory diseases. Copyright © 2010 John Wiley & Sons, Ltd.

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