Development of an in vivo gene mutation assay using the endogenous Pig-A gene: I. Flow cytometric detection of CD59-negative peripheral red blood cells and CD48-negative spleen T-cells from the rat

Development of an in vivo gene mutation

Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants

✍ Daishiro Miura; Vasily N. Dobrovolsky; Roberta A. Mittelstaedt; Yoshinori Kasaha 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 365 KB 👁 1 views

## Abstract We previously reported that rat spleen T‐cells and peripheral red blood cells that are deficient in glycosylphosphatidylinositol (GPI) synthesis [presumed mutants for the phosphatidylinositol glycan complementation group A gene (__Pig‐A__)] could be detected by flow cytometry (FCM) as c