Development of an immunoaffinity column and an indirect immunoassay with a biotin-streptavidin detection system for aflatoxin M1 in milk
✍ Scribed by C. De Boevere; C. Van Peteghem
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 372 KB
- Volume
- 275
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A sensmve and rapId screemng method for the estnnation of atlatoxm M, (APM,) 111 mdk has been developed Mdk samples were first purd'ied by mnnunoaffin~ty chromatography (IAC) usmg polyclonal ant&o&es ram~I m rabbits agamst ailatoxm MI-bovme serum albumm &FM,-BSA) and coupled to an a-ted sepharose matnx. The eluate was analyzed III an mdnect competitwe streptawdm-blotm modtied enzyme bnked mummoassay (EIA) Mnzrotltre plates were coated wrth APM,-BSA that competes mth the analyte m the sample for bmdmg with the blotmylated a&body Bound blohnylated antlbody was detected usmg a streptavldm blotmylated horseradish peromdase complex The llrmt of dete&on of the EXA IS 2 ng kg-' By combmatlon of IAC and EIA, spiked nulk samples were analyzed In conclusion, IAC-EIA 1s a sensltwe and rapld method for the estimation of APM, m nulk.