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Development of an HTS assay for EPHX2 phosphatase activity and screening of nontargeted libraries

โœ Scribed by Morisseau, Christophe; Sahdeo, Sunil; Cortopassi, Gino; Hammock, Bruce D.

Book ID
122030133
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
931 KB
Volume
434
Category
Article
ISSN
0003-2697

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โœ Shinya Mitsuhashi; Hiroshi Shima; Kunimi Kikuchi; Keiichi Igarashi; Rei Hatsuse; ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 96 KB

Despite the physiological importance of serine/threonine protein phosphatase type 2B (PP2B/calcineurin), an accurate assay method of PP2B in crude tissue extracts has not been established. By using recombinant protein phosphatase inhibitor-1 as a substrate and ascorbic acid as an antioxidant, we dev