Development of an HPLC method for the determination of salidroside in beagle dog plasma after administration of salidroside injection: Application to a pharmacokinetics study

✍ Scribed by Yu Mao; Xinrong Zhang; Xiaodong Zhang; Guocai Lu

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 365 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700273

✦ Synopsis

Abstract

A simple RP‐HPLC method was established for the determination of salidroside in dog plasma. Salidroside is one of the most active ingredients of Rhodiola L. The method had within‐run precision values in the range of ± 2.3 to ± 9.1% (n = 5) and between‐run precision in the range of ± 3.2 to ± 9.8%. A simple protein precipitation for salidroside extraction was processed using ACN at precipitant‐to‐plasma volume ratio (P–P ratio) of 3:2. The extraction recoveries of salidroside at seven concentrations were higher than 63.2%. There was a linear relationship between chromatographic area and concentration over the range of 0.83–520 μg/mL for salidroside in plasma (R = 0.9926). The LOQ (S/N = 10) of the method was 0.83 μg/mL. The method was applied in a study of the pharmacokinetics of salidroside injection in six beagle dogs. The major pharmacokinetic parameters of C~max~, AUC~0–24~, AUC~0–∞~, and t~1/2~ of salidroside in beagle dogs after i.v. administration of a single 75 mg/kg (5 mL/kg) dose were 96.16 ± 8.59 μg/mL, 180.3 ± 30.6 μg·h/mL, 189.3 ± 32.1 μg·h/mL, and 2.006 ± 0.615 h, respectively.